Evolutionary and Functional Genomics of Bacteria from the Cold Deep Sea
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Published on 2007 by ProQuest
Chapter 2 describes the development of three new vectors for use in Photobacterium profundum and other Gram-negative bacteria. The first one (pEE3) is a suicide vector for insertional inactivation of genes. The second one is a general purpose cloning vector (pFL122) based on the broad-host-range replicon RSF1010. The third one is a broad-host-range vector carrying an arabinose-inducible promoter to use for gene expression studies.
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